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Analyte molecules partition concerning a liquid stationary stage and also the eluent. Equally as in hydrophilic conversation chromatography (HILIC; a sub-method in just HPLC), this method separates analytes based on variations inside their polarity. HILIC most often uses a bonded polar stationary stage plus a cell phase manufactured mainly of aceto
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-hydroxybenzoic acid (PH) over a nonpolar C18 column subject into a maximum Examination time of 6 min. The shaded spots characterize locations wherever a separation is impossible, With all the unresolved solutes determined.Wherever N is the quantity of theoretical plates, L could be the duration on the column, and H is the height equivalent to a th